Quantitative Thin-Layer Chromatography

Download or read book Quantitative Thin-Layer Chromatography written by Bernd Spangenberg and published by Springer Science & Business Media. This book was released on 2011-01-03 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.