A BEGINNER’S GUIDE TO PRACTICAL MOLECULAR ENTOMOLOGY

Download or read book A BEGINNER’S GUIDE TO PRACTICAL MOLECULAR ENTOMOLOGY written by RAMANA NARAVA and published by American Academic Press. This book was released on 2020-12-01 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual describes laboratory procedures, equipment uses, preparation of reagents, DNA and RNA isolation, electrophoresis, blotting and hybridisation, molecular markers, PCR, DNA barcodes, sequencing methods, gene expression studies, and RNAi. Each chapter consists of an outline of the principles concerned, a schematic explanation of the procedure with elaborated protocols. The book is main geared for students who are relatively new to practical molecular entomology and therefore the emphasis is on making techniques accessible to those individuals. As the author admits, there is a degree of repetition, what this implies is that the majority of the chapters are basically complete and standard thus, there's no looking around for recipes, however in fact it conjointly pushes up the dimensions. The chapters written in this book gives the background information which can be allowed to modify at their end and design new protocols.