Investigating the Effects of Nucleosome Remodeling Factor Knockdown on Anti-tumor Immunity

Download or read book Investigating the Effects of Nucleosome Remodeling Factor Knockdown on Anti-tumor Immunity written by Mark G. Roberts and published by . This book was released on 2016 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: The nucleosome remodeling factor (NURF) is a chromatin remodeling complex involved in early animal development and is implicated in a number of cancers. In previous work, knockdown of NURF's largest subunit, BPTF, resulted in diminished tumor growth in mouse cancer cell lines. Other studies in our lab demonstrated increased activation of T-lymphocytes into BPTF KD tumors. In order to examine if this approach has any therapeutic potential, this work investigates the effects of BPTF knockdown in established tumors by using recombinant adenoviruses (rAd), as well as observe the way the immune system interacts with BPTF knockdown cells, both in vivo by flow cytometry and in culture with cytotoxicity assays.