Microinjection

Download or read book Microinjection written by Juan Carlos Lacal and published by Birkhäuser. This book was released on 2013-12-01 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system.